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W. G. REGESTER, M. D.,
State Vaccine Agent,
S. W. Corr. Fayette and Calhoun Streets,
BALTIMORE, MD.

Directions for the Preservation and Use of Vaccine Lymph.

If practicable, the slips should all be used immediately upon opening the package. If,
however, it is necessary to keep some of them for future use, they should be removed from
the package and put into a perfectly dry bottle, provided with a closely-fitted cork or rubber
(not glass) stopper.

In vaccinating with quill-slips, first moisten the charge of lymph (on the convex surface of
the slip, at the square cut end) with a drop of cold water. The longer the lymph has been kept,
the more necessary is this proceeding. Lay the slip on a table, with its moistened side up.
Then proceed to make the abrasion, using a sharp lancet. The skin should not be punctured,
cut or scratched, but it should be scraped so as to remove the outer layer of the epidermis,
without wounding the derma. The manipulation is the same as is used in erasing ink-marks
from paper. When the abrasion has become moist (no matter whether or not it shows the color
of blood), the moistened lymph should be thoroughly applied to it by rubbing it briskly with
the charged end of the quill-slip for a full minute or more. The arm should be kept bare for
ten minutes, but no plaster or other dressing need be applied.

[OVER.]